詳細(xì)說(shuō)明
Lolina A/SAddress: Sindalsvej 30 8240 Risskov Danmark
Email: Info@lolina.dk
Product Specification
Product | Lolina? Bst Plus DNA Polymerase (40 U/μL) |
Catalog No. | NaM702001 |
Storage conditions | Store at -25 ~ -15℃, valid for 2 year. |
Enzyme Storage Buffer | 10 mM Tris-HCl, 50 mM KCl, 0. 1 mM EDTA, 1 mM DTT, 0. 1% Triton X-100, 50% Glycerol pH 7.5 @ 25°C |
Heat Inactivation | 85°C, 5 min |
Unit Definition | 1 unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of dNTPs into acid-insoluble precipitate under the reaction conditions of 65°C for 30 minutes. |
Product Applications | This product is suitable for various isothermal amplification reactions such as LAMP, CPA, RCA, etc. |
Product description
Lolina? Bst Plus DNA Polymerase is derived from Thermophilic Geobacillus sp DNA Polymerase I,
lacking 5 ′-3′ exonuclease activity. The enzyme has stronger 5 ′-3′ DNA polymerase activity, strand displacement activity and dUTP tolerance, which is more suitable for anti-pollution isothermal amplification reactions, such as LAMP, CPA, etc.
CAT | Components Name | Size | |
8,000 U | 40,000 U | ||
NaM702001-A | Lolina? Bst Plus DNA Polymerase (40 U/μL) | 200 μL | 1 mL |
NaM702001-B | 10× Lolina? Bst Plus DNA Polymerase Buffer | 1 mL | 3 × 1 mL |
NaM702001-C | 100 mM MgSO4 | 1 mL | 3 × 1 mL |
Instructions
1.Reaction System
Components | Volume (μL) | Final Concentration |
10x Reaction Buffer | 2.5 | 1 x |
100 mmol/L MgSO4 | 0.75 | 3 mmol/L+ 2 mmol/L in buffer = 5 mmol/L |
dNTP Mix (25 mmol/L each) | 1.4 | 1.4 mmol/L each |
dUTP (25 mmol/L) (optional) | 1.4 | 1.4 mmol/L |
UDGase (1U/μl) (optional) | 1 | 0.04 U |
DNA | 10 ng – 1μg | - |
10x Primers | 2.5 | - |
Lolina? Bst Plus DNA Polymerase (40 U/μl) | 1 | 1.6 U/μl |
ddH2O | to 25 |
[Note]:
a) The amount of Lolina? Bst Plus DNA Polymerase can be adjusted and optimized according to different experiments.
b) 10× Reaction Buffer: 200 mM Tris-HCl, 500 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% Tween-20, pH 8.8 @ 25°C.
c) The Mg2+ concentration can be adjusted between 4 – 10 mM according to different experiments.
d) 10× Primers: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B each.
Temp. | Time | Function |
25 - 37°C | 5 – 10 min | Degradation of templates containing uracil (optional) |
65°C | 30 – 60 min | Reaction |
85°C | 5 min | Inactivation |
Notes
1. The enzyme should be stored in a cooler or on ice during use and immediately placed at -20°C after use. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
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